PCI-e NVME Expansion card works in 1 PCI-e 16x slot but not the second

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I am trying to use a Rivo Dual M.2 SATA III and M2 NVME to PCIe 3.0 X4 Adapter in my Gigabyte B550M DS3H AC motherboard.

The problem is that when I plug the Rivo card into the second PCI-e x16 slot the NVME is not detected. There is a RTX 3060ti in the first slot.

When I swap the cards around and put the Rivo in the top slot and the GPU in the bottom slot. Everything works. The GPU works and the NVME in the Rivo adapter works.

I have had a look in the bios and have tried adjusting PCI-e settings such as what gen the slots are and PCI-e bifurcation but nothing has gotten the Rivo detected in the second slot.

Has anyone ever come across this before?
 
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