I don't think it was a low light problem. I was directly under a pair of 5ft fluorescent tubes and the exposure was 1/160 f1.4 @ ISO400.
I wasn't taking a serious photograph, I was just trying to demonstrate the 5Dmk2's micro-focus adjustment feature to a friend.
It was a nice, easy to focus on, high contrast subject. I was using a good tripod and timer shutter release. I was getting random results suggesting anywhere between -5 and +2 adjustment.
The lens used was my Canon 50mm f1.4 USM which I have previous, in good daylight, easily determinded needed a +2 adjustment.