PTFE tape or not ?

It they are all brand new, the o-rings will do on their own.

O-rings can perish over time and should ideally be replaced each time you refit them.
When I am re-using barbs I use PTFE with the o-rings as I haven't found a source of replacement o-rings to fit.
 
I've used those compression type fittings throughout, without PTFE tape and haven't had a leak yet. They are quite tough to tighten up so I would have struggled with PTFE anyway.

Mind you they all have an O ring at the base.
 
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