Nope, it will look to SATA 1 for a Boot drive 1st though.

, but because i am a odd kid and when i got my 2 x 74gb Raptors and switched the boot from my Seagate Baracude to the 2 raptors at Raid 0, i had to have the Raptors in Sata 1 and 2, just because i am an odd kid 