PCIe-4 in PCIe-5 slots

[sargan]

I have a 2 TB PCIe-5 drive in the main M2 drive slot - which is PCIe-50
I also have a secondary M.2 drive which is PCIe-4.0 as my motherboard has 2 x PCIe-5.0 slots and 2 x PCI3-4.0 slots
Will a PCIe-4 drive work any faster on a PCIe-5 slot (as its direct connect to CPU) than if its in a PCIe4.0 slot which goes via a controller module.

 

[sargan]

just an interest Q

 

[sargan]

The background is M.2 slot 2 (PCIe-5) not working, tested same M.2 module in slots 3&4 (PCIe-4) and it works fine.
Replacement motherboard on its way.