I would think the raid would have to be built first. You could try creating an image of the single drive, creating the raid array and ghosting back onto the raid0, but cant be sure if it will work.
If you mean can you add a hard drive to an existing drive to create a Raid0 array e.g. you have one drive and want to start striping the data across that one and a new drive then the answer is not without building the array and formatting.
You could try wizardmaxx's plan if you have a spare hard drive or the data is small enough to fit onto a DVD so that you can Ghost it back on but remember to give yourself the best chance you will need to install the Raid drivers into the existing Windows install so that it at least has a hope of being picked up on the new array.
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