From what I can tell from the manual (assuming I'm reading the right one) is that the blue PCIe 16x slot always runs at 16x. The black slot, despite being 16x sized, only runs up to 4x speed because of the limited number of lanes available from the P35 chipset.
What I will suggest is that you avoid PCIe 1x cards, the P35 chipset is only PCIe rev 1.1 so a 1x card is limited to 250Mb/s so will bottleneck a decent SATA3 SSD.