3/8" Tygon. More expensive, more flexible. You can use it with 1/2" barbs.
use metal barbs over plastic. More rubust (wont crimp easiliy) and will last longer when dealing with low temperatures (like if your PC is outside or using a Waterchiller)
O rings do the job. No need for PTFE tape nowadays, although using both could be a wise preventative measure.
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