So if I put an OS on the last partition and first the one on the last would be quite abit slower? And having any amount of partitions then will not cause any sort of slowdown to the performance and loading of my OS?
You drive becomes a bigger bottleneck if you're accessing multiple partitions on the same drive at the same time.
Probably not that a big deal unless you're doing a lot of multitasking or partition to partition file transfers.
I doubt you'll notice to be honest.