Today's work was to double check the shower tray fitting - I moved the joist over slightly as it was just floating on an RSJ (!) but it still wouldn't quite work with backer board behind it, so I'm going to have to bring the board down to the tray otherwise the joist will just be in the way of the trap. Once the tray was lined up, I marked centres for the valve and rain head.
Also spent a few hours reinforcing the floor with 2x4, focused around the tray area. It's made a huge difference to the integrity of the floor; almost no wobble now so I'm confident that ply / no more ply + tile backer will be a good solid floor which combined with S2 adhesive, should prevent any tile or grout cracks.
Next big job is first fix plumbing, which I've been procrastinating over for far too long in true DIY fashion.
Just remember that your stud wallvused to sit on top of some supported timber and now you've cut off one side so I'd get some noggins into that stud wall.
Yes I'd clip all the pipework (both the wastes, hot/cold and heating.
Get yourself some of these if you want to make it easier than making timbers for it.
For insulation if its a heated room below then it's not really needed. I would add it for sound insulation more than anything else.
I also wouldn't bother with electrical floor heating, it's expensive to run.
This bathroom is directly above an unheated garage so although it will have XPS board, I'm definitely going to insulate between the joists as it's the last time to do so until the bathroom is next replaced in 15 (?!) years, so I might as well do it right now.
Electric UFH - this is just for warming the floor and drying up water spills, rather than being the primary source of heat for the room. It's very popular and having had it before, I wouldn't be without it in a bathroom. Cost to run is peanuts vs. the benefit, for me anyway.
Thanks for the thoughts so far.